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VDAC1 antibody [N1C2]

GTX114187
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetVDAC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    VDAC1 antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.46 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7416
  • Target name
    VDAC1
  • Target description
    voltage dependent anion channel 1
  • Target synonyms
    outer mitochondrial membrane protein porin 1; plasmalemmal porin; PORIN; porin 31HL; porin 31HM; sperm binding protein 1a; VDAC-1; voltage-dependent anion-selective channel protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21796
  • Protein Name
    Voltage-dependent anion-selective channel protein 1
  • Scientific Description
    This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Human neurotropic polyomavirus, JC virus, agnoprotein targets mitochondrion and modulates its functions. Saxena R et al., 2021 Jan 15, Virology
    Read more
  • Ameliorative Effect of Sodium Selenite on Silver Nanoparticles-Induced Myocardiocyte Structural Alterations in Rats. Ma W et al., 2020, Int J Nanomedicine
    Read more
  • Absence of uncoupling protein 3 at thermoneutrality influences brown adipose tissue mitochondrial functionality in mice. Silvestri E et al., 2020 Nov, FASEB J
    Read more
  • The SMAC mimetic LCL161 is a direct ABCB1/MDR1-ATPase activity modulator and BIRC5/Survivin expression down-regulator in cancer cells. Chang YC et al., 2020 Aug 15, Toxicol Appl Pharmacol
    Read more
  • Silver Nanoparticle Exposure Causes Pulmonary Structural Damage and Mitochondrial Dynamic Imbalance in the Rat: Protective Effects of Sodium Selenite. Ma W et al., 2020, Int J Nanomedicine
    Read more
  • Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species. Lee CW et al., 2019 Aug, J Cell Physiol
    Read more
  • Glycyrrhizic acid induces human MDA-MB-231 breast cancer cell death and autophagy via the ROS-mitochondrial pathway. Lin SC et al., 2018 Feb, Oncol Rep
    Read more
  • The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKbeta/AMPK pathways. Chen X et al., 2017 Aug, Redox Biol
    Read more
  • The PARP inhibitor olaparib enhances the cytotoxicity of combined gemcitabine, busulfan and melphalan in lymphoma cells. Valdez BC et al., 2017 Nov, Leuk Lymphoma
    Read more
  • Extracellular vesicles released by hepatocytes from gastric infusion model of alcoholic liver disease contain a MicroRNA barcode that can be detected in blood. Eguchi A et al., 2017 Feb, Hepatology
    Read more

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Figure 1. Western blot analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Porin/VDAC1 antigen affinity purified polyclonal antibody (Catalog # A01168-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Porin/VDAC1 at approximately 34 kDa. The expected band size for Porin/VDAC1 is at 31 kDa.
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