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Vegfc Antibody Pair

CSB-EAP07725
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityAvian, Bovine, Canine, Equine, Human, Mouse, Porcine
TargetVegfc
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Overview

  • Supplier
    Cusabio
  • Product Name
    Vegfc Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID22341
  • Target name
    Vegfc
  • Target description
    vascular endothelial growth factor C
  • Target synonyms
    VEGF-C, vascular endothelial growth factor C, VRP, flt4 ligand, flt4-L, vascular endothelial growth factor-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP97953
  • Protein Name
    Vascular endothelial growth factor C
  • Reactivity
    Avian, Bovine, Canine, Equine, Human, Mouse, Porcine
  • UNSPSC
    41116163

FAQ

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Figure 1. Western blot analysis of Vegfc using anti-Vegfc antibody (A00623-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vegfc antigen affinity purified polyclonal antibody (Catalog # A00623-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vegfc at approximately 46 kDa. The expected band size for Vegfc is at 46 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:400 overnight at 4°C, DAB staining.
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