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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA025855ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA025855ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA025855ESR1HU at dilution of 1:100

VIL1 Antibody

CSB-PA025855ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetVIL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    VIL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7429
  • Target name
    VIL1
  • Target description
    villin 1
  • Target synonyms
    D2S1471; VIL; villin-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09327
  • Protein Name
    Villin-1
  • Scientific Description
    Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human colon, gallbladder, kidney and liver using Anti-VIL1 antibody HPA006884 (A) shows similar protein distribution across tissues to independent antibody HPA006885 (B).
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Lane 1: Mouse Colon; Lane 2: Human Small Intestine; Lane 3: Human Colon; Lane 4: Rat Kidney; Probed with Villin-1 (5C1) Monoclonal Antibody (bsm-54212R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Figure 1. Western blot analysis of Villin using anti-Villin antibody (PB9457). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Intestine Tissue Lysate at 50ug Lane 2: Mouse Kidney Tissue Lysate at 50ug Lane 3: RH35 Whole Cell Lysate at 40ug Lane 4: HEPG2 Whole Cell Lysate at 40ug, Lane 5: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Villin antigen affinity purified polyclonal antibody (Catalog # PB9457) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Villin at approximately 93 kDa. The expected band size for Villin is at 93 kDa.
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