Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220701_WB_M_R_22070501_818.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220701_IHC-P_M_22071823_395.webp "Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Vimentin antibody [HL1506] detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220701_IHC-P_22071823_281.webp "Vimentin antibody [HL1506] detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220812_WB_competitor_watermark_22081423_808.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220812_WB_B_22081423_799.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Wild-type (WT) and Vimentin knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20220819_WB_KO_watermark_22082402_854.webp "Wild-type (WT) and Vimentin knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Vimentin antibody [HL1506] detects Vimentin protein at cytoskeleton and cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX636980/GTX636980_T-44676_20220916_ICC_IF_22102723_567.webp "Vimentin antibody [HL1506] detects Vimentin protein at cytoskeleton and cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20221228_IHC-P_multiple_M_22122901_907.webp "Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat lymph node. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636980/GTX636980_44725_20230331_IHC-P_Cat_23032819_622.webp "Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat lymph node. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin antibody [HL1506]
GTX636980
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityFeline, Human, Mouse, Rat
TargetVIM
Overview
- SupplierGeneTex
- Product NameVimentin antibody [HL1506]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1506
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7431
- Target nameVIM
- Target descriptionvimentin
- Target synonymsvimentin, epididymis secretory sperm binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP08670
- Protein NameVimentin
- Scientific DescriptionThis gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
- ReactivityFeline, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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