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Vimentin Antibody

LS-C40194
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityCanine, Goat, Hamster, Human, Porcine, Rat
TargetVIM
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Vimentin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Flow Cytometry: 1:50-1:100. Immunohistochemistry (frozen sections): 1:50-1:100. Avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Western Blot: 1:50-1:500. Optimum dilutions to be determined by researcher. Hybridoma: SP2/0-Ag14 mouse myeloma with spleen cells from a BALB/c mouse immunized with cytoskeletal vimentin extract of calf lens.. Flo (1:50 - 1:100), ICC, IHC, IHC-Fr, WB (1:50 - 1:500) Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Flow Cytometry: 1:50-1:100. Immunohistochemistry (frozen sections): 1:50-1:100. Avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Western Blot: 1:50-1:500. Optimum dilutions to be determined by researcher. Hybridoma: SP2/0-Ag14 mouse myeloma with spleen cells from a BALB/c mouse immunized with cytoskeletal vimentin extract of calf lens.
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7431
  • Target name
    VIM
  • Target description
    vimentin
  • Target synonyms
    vimentin, epididymis secretory sperm binding protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Canine, Goat, Hamster, Human, Porcine, Rat
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Vimentin using anti-Vimentin antibody (PB9359). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human MCF-7 whole cell lysates, Lane 9: rat ovary tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (Catalog # PB9359) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa.
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