More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Vimentin Antibody

Vimentin Antibody

LS-C40201
LifeSpan BioSciences
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey
TargetVIM
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Vimentin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Immunohistochemistry (paraffin embedded sections), and Immunocytochemistry ( in vitro fixed in ice cold absolute methanol or acetone). Immunohistochemistry: 1:500-1:2000. Immunocytochemistry: 1:100-1:200.. ICC (1:100 - 1:200), IHC, IHC-P Suitable for use in Immunohistochemistry (paraffin embedded sections), and Immunocytochemistry ( in vitro fixed in ice cold absolute methanol or acetone). Immunohistochemistry: 1:500-1:2000. Immunocytochemistry: 1:100-1:200.
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7431
  • Target name
    VIM
  • Target description
    vimentin
  • Target synonyms
    vimentin, epididymis secretory sperm binding protein
  • Host
    Mouse
  • Isotype
    IgM
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biomatik
Vim Polyclonal AntibodyCAC07060
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetVIM
  • SizePrice
Product group Antibodies
RayBiotech
Anti-VIM Antibody130-10171
ApplicationsWestern Blot, ELISA
  • SizePrice
Immunohistochemical staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.
Product group Antibodies
Atlas Antibodies
Anti-VIM AntibodyAMAB90516
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetVIM
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Vimentin [1433]AB01640-1.9-C
ApplicationsImmunoFluorescence
ReactivityCanine, Human, Rat
TargetVIM
  • SizePrice
Product group Antibodies
MedChem Express
Vimentin Antibody (YA011, Rabbit)HY-P80371
TargetVIM
  • SizePrice
Product group Antibodies
References
Everest Biotech
Goat anti-VimentinEB11207
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetVIM
  • SizePrice
Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain tissue, diluted at 1:2000.
Product group Antibodies
Cusabio
VIM Monoclonal AntibodyCSB-MA000319
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetVIM
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
Vimentin Antibody (Preservative Free)LS-C343535
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetVIM
  • SizePrice
Product group Antibodies
Nordic-MUbio
Mouse anti VimentinMUB1902P-CE/IVD
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityChicken, Human, Mammals, Rat, Sheep
TargetVIM
  • SizePrice
Figure 1. Western blot analysis of Vimentin using anti-Vimentin antibody (PB9359). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human MCF-7 whole cell lysates, Lane 9: rat ovary tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (Catalog # PB9359) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa.
Product group Antibodies
Boster Bio
Anti-Vimentin Antibody Picoband(r)PB9359-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetVIM
  • SizePrice