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Image submitted by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining and detection.
Image submitted by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining and detection.
Image submitted by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining and detection.

Vimentin Polyclonal Antibody

BS-0756R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetVIM
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Overview

  • Supplier
    Bioss
  • Product Name
    Vimentin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7431
  • Target name
    VIM
  • Target description
    vimentin
  • Target synonyms
    vimentin, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08670
  • Protein Name
    Vimentin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Jin L, Zhang J, Deng Z, et al. Mesenchymal stem cells ameliorate myocardial fibrosis in diabetic cardiomyopathy via the secretion of prostaglandin E2. Stem Cell Res Ther. 2020,11(1):122. doi: 10.1186/s13287-020-01633-7
    Read this paper
  • Muhammad T, Sakhawat A, Khan AA, et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020,146(4):861-874. doi: 10.1007/s00432-020-03157-2
    Read this paper
  • Yin X, Li Q, Wei H, et al. Zinc oxide nanoparticles ameliorate collagen lattice contraction in human tenon fibroblasts. Arch Biochem Biophys. 2019,669:1-10. doi: 10.1016/j.abb.2019.05.016
    Read this paper
  • Zhou S, Chen H, Yuan P, et al. Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B. Biochem Biophys Res Commun. 2019,514(3):893-900. doi: 10.1016/j.bbrc.2019.05.036
    Read this paper
  • Tarique I, Vistro WA, Bai X, et al. LIPOPHAGY: a novel form of steroidogenic activity within the LEYDIG cell during the reproductive cycle of turtle. Reprod Biol Endocrinol. 2019,17(1):19. doi: 10.1186/s12958-019-0462-2
    Read this paper
  • Ye T, Yang M, Huang D, et al. MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer. J Exp Clin Cancer Res. 2019,38(1):55. doi: 10.1186/s13046-019-1074-6
    Read this paper
  • Wang JY, Wu T, Ma W, et al. Expression and clinical significance of autophagic protein LC3B and EMT markers in gastric cancer. Cancer Manag Res. 2018,10:1479-1486. doi: 10.2147/CMAR.S164842
    Read this paper
  • Zhou Q, Zhou Y, Liu X, et al. GDC-0449 improves the antitumor activity of nano-doxorubicin in pancreatic cancer in a fibroblast-enriched microenvironment. Sci Rep. 2017,7(1):13379. doi: 10.1038/s41598-017-13869-0
    Read this paper
  • Park S, Sung Y, Jeong J, et al. Critical roles of hMAGEA2 in induced pluripotent stem cell pluripotency, proliferation, and differentiation. Cell Biochem Funct. 2017,35(7):392-400. doi: 10.1002/cbf.3286
    Read this paper
  • Tanos T, Saibene AM, Pipolo C, et al. Isolation of putative stem cells present in human adult olfactory mucosa. PLoS One. 2017,12(7):e0181151. doi: 10.1371/journal.pone.0181151
    Read this paper

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Figure 1. Western blot analysis of Vimentin using anti-Vimentin antibody (PB9359). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human MCF-7 whole cell lysates, Lane 9: rat ovary tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (Catalog # PB9359) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa.
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