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ProductsBack/Vimentin(LN-6), 1mg/mL [26628-22-8]
Vimentin(LN-6), 1mg/mL [26628-22-8]

Vimentin(LN-6), 1mg/mL [26628-22-8]

BNUM0060
Biotium
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Feline, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetVIM
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Overview

  • Supplier
    Biotium
  • Product Name
    Vimentin(LN-6), 1mg/mL [26628-22-8]
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • CAS Number
    26628-22-8
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    LN-6
  • Concentration
    1 mg/ml
  • Gene ID7431
  • Target name
    VIM
  • Target description
    vimentin
  • Target synonyms
    vimentin, epididymis secretory sperm binding protein
  • Host
    Mouse
  • Isotype
    IgM
  • Protein IDP08670
  • Protein Name
    Vimentin
  • Scientific Description
    This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).
  • Source
    Animal
  • Reactivity
    Bovine, Feline, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of Vimentin using anti-Vimentin antibody (PB9359). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human MCF-7 whole cell lysates, Lane 9: rat ovary tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (Catalog # PB9359) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa.
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