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WB analysis of COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA which were either untreated (Lane 1 and 6) or treated with vanadate for 24 hr (Lane 2-5 and 7-10) using GTX54517 Vinculin (phospho Tyr100) antibody (left panel) or Vinculin antibody (right panel).
WB analysis of COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA which were either untreated (Lane 1 and 6) or treated with vanadate for 24 hr (Lane 2-5 and 7-10) using GTX54517 Vinculin (phospho Tyr100) antibody (left panel) or Vinculin antibody (right panel).
WB analysis of COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA which were either untreated (Lane 1 and 6) or treated with vanadate for 24 hr (Lane 2-5 and 7-10) using GTX54517 Vinculin (phospho Tyr100) antibody (left panel) or Vinculin antibody (right panel).

Vinculin (phospho Tyr100) antibody

GTX54517
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityChicken, Human, Mouse, Rat
TargetVCL
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Overview

  • Supplier
    GeneTex
  • Product Name
    Vinculin (phospho Tyr100) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:250. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7414
  • Target name
    VCL
  • Target description
    vinculin
  • Target synonyms
    CMD1W; CMH15; epididymis luminal protein 114; epididymis secretory sperm binding protein; HEL114; metavinculin; meta-vinculin; MV; MVCL; vinculin; vinculin isoform meta-VCL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18206
  • Protein Name
    Vinculin
  • Scientific Description
    Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
  • Reactivity
    Chicken, Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203