Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Visfatin antibody [4D5]
GTX50049
Overview
- SupplierGeneTex
- Product NameVisfatin antibody [4D5]
- Delivery Days Customer9
- Application Supplier NoteWe recommend the following starting dilutions:Western Blot: Use at 1:1,000~ 2,000.Optimal working concentrations should be determined experimentally by the end user.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4D5
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID10135
- Target nameNAMPT
- Target descriptionnicotinamide phosphoribosyltransferase
- Target synonyms1110035O14Rik; NAmPRTase; nicotinamide phosphoribosyltransferase; PBEF; PBEF1; pre-B cell-enhancing factor; pre-B-cell colony-enhancing factor 1; VF; VISFATIN
- HostMouse
- IsotypeIgG1
- Protein IDP43490
- Protein NameNicotinamide phosphoribosyltransferase
- Scientific DescriptionThis gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203