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ProductsBack/Vitamin D Receptor / VDR Antibody (aa377-402)

Vitamin D Receptor / VDR Antibody (aa377-402)

LS-C407668
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVDR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Vitamin D Receptor / VDR Antibody (aa377-402)
  • Delivery Days Customer
    23
  • Application Supplier Note
    WB: The detection limit for VDR is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.. IHC, IHC-P (0.5 - 1 µg/ml), WB (0.1 - 0.5 µg/ml) WB: The detection limit for VDR is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7421
  • Target name
    VDR
  • Target description
    vitamin D receptor
  • Target synonyms
    NR1I1, PPP1R163, vitamin D3 receptor, 1,25-dihydroxyvitamin D3 receptor, nuclear receptor subfamily 1 group I member 1, protein phosphatase 1, regulatory subunit 163, vitamin D (1,25- dihydroxyvitamin D3) receptor
  • Host
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Vitamin D Receptor/VDR using anti-Vitamin D Receptor/VDR antibody (A00210-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitamin D Receptor/VDR antigen affinity purified polyclonal antibody (Catalog # A00210-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitamin D Receptor/VDR at approximately 60 kDa. The expected band size for Vitamin D Receptor/VDR is at 60 kDa.
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