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ProductsBack/VMAT2 antibody [9E11]
FACS analysis of HEK293T cells transfected with either VMAT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83635 VMAT2 antibody [9E11].
FACS analysis of HEK293T cells transfected with either VMAT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83635 VMAT2 antibody [9E11].
FACS analysis of HEK293T cells transfected with either VMAT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83635 VMAT2 antibody [9E11].

VMAT2 antibody [9E11]

GTX83635
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC18A2
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Overview

  • Supplier
    GeneTex
  • Product Name
    VMAT2 antibody [9E11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6571
  • Target name
    SLC18A2
  • Target description
    solute carrier family 18 member A2
  • Target synonyms
    PKDYS2, SVAT, SVMT, VAT2, VMAT2, synaptic vesicular amine transporter, monoamine neurotransmitter transporter, solute carrier family 18 (vesicular monoamine transporter), member 2, synaptic vesicle amine transporter, brain, synaptic vesicle monoamine transporter, brain, vesicle monoamine transporter type 2, vesicle monoamine/H+ antiporter, vesicular amine transporter 2, vesicular monoamine transporter 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ05940
  • Protein Name
    Synaptic vesicular amine transporter
  • Scientific Description
    Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VMAT2/SLC18A2 using anti-VMAT2/SLC18A2 antibody (A02232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VMAT2/SLC18A2 antigen affinity purified polyclonal antibody (Catalog # A02232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VMAT2/SLC18A2 at approximately 75 kDa. The expected band size for VMAT2/SLC18A2 is at 56 kDa.
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