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ProductsBack/VMAT2 (SLC18A2) Mouse Monoclonal Antibody [Clone ID: OTI9E11]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SLC18A2 (Cat# RC221342, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC18A2 (Cat# TA500506). Positive lysates [LY401066] (100ug) and [LC401066] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SLC18A2 (Cat# RC221342, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC18A2 (Cat# TA500506). Positive lysates [LY401066] (100ug) and [LC401066] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SLC18A2 (Cat# RC221342, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC18A2 (Cat# TA500506). Positive lysates [LY401066] (100ug) and [LC401066] (20ug) can be purchased separately from OriGene.

VMAT2 (SLC18A2) Mouse Monoclonal Antibody [Clone ID: OTI9E11]

CF500506
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC18A2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    VMAT2 (SLC18A2) Mouse Monoclonal Antibody [Clone ID: OTI9E11]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI9E11
  • Gene ID6571
  • Target name
    SLC18A2
  • Target description
    solute carrier family 18 member A2
  • Target synonyms
    PKDYS2, SVAT, SVMT, VAT2, VMAT2, synaptic vesicular amine transporter, monoamine neurotransmitter transporter, solute carrier family 18 (vesicular monoamine transporter), member 2, synaptic vesicle amine transporter, brain, synaptic vesicle monoamine transporter, brain, vesicle monoamine transporter type 2, vesicle monoamine/H+ antiporter, vesicular amine transporter 2, vesicular monoamine transporter 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ05940
  • Protein Name
    Synaptic vesicular amine transporter
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SLC18A2 mouse monoclonal antibody, clone OTI9E11 (formerly 9E11)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of VMAT2/SLC18A2 using anti-VMAT2/SLC18A2 antibody (A02232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VMAT2/SLC18A2 antigen affinity purified polyclonal antibody (Catalog # A02232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VMAT2/SLC18A2 at approximately 75 kDa. The expected band size for VMAT2/SLC18A2 is at 56 kDa.
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