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ProductsBack/VPRBP (C-8): m-IgG2b BP-HRP Bundle

VPRBP (C-8): m-IgG2b BP-HRP Bundle

SC-549089
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetDCAF1
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    VPRBP (C-8): m-IgG2b BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID9730
  • Target name
    DCAF1
  • Target description
    DDB1 and CUL4 associated factor 1
  • Target synonyms
    DDB1- and CUL4-associated factor 1; HIV-1 Vpr-binding protein; protein VPRBP; RIP; serine/threonine-protein kinase VPRBP; Vpr (HIV-1) binding protein; Vpr-binding protein; VPRBP; vpr-interacting protein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VPRBP/DCAF1 using anti-VPRBP/DCAF1 antibody (A31776-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPRBP/DCAF1 antigen affinity purified polyclonal antibody (Catalog # A31776-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPRBP/DCAF1 at approximately 169 kDa. The expected band size for VPRBP/DCAF1 is at 169 kDa.
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