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ProductsBack/WDR77 antibody [8A10-C10-E8]
ICC/IF analysis of HeLa cells using WDR77 antibody at a dilution of 1:100.
ICC/IF analysis of HeLa cells using WDR77 antibody at a dilution of 1:100.
ICC/IF analysis of HeLa cells using WDR77 antibody at a dilution of 1:100.

WDR77 antibody [8A10-C10-E8]

GTX49156
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetWDR77
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Overview

  • Supplier
    GeneTex
  • Product Name
    WDR77 antibody [8A10-C10-E8]
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8A10-C10-E8
  • Conjugate
    Unconjugated
  • Gene ID79084
  • Target name
    WDR77
  • Target description
    WD repeat domain 77
  • Target synonyms
    androgen receptor cofactor p44; HKMT1069; MEP50; MEP-50; methylosome protein 50; Nbla10071; p44; p44/Mep50; testis tissue sperm-binding protein Li 44a; WD repeat-containing protein 77
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9BQA1
  • Protein Name
    Methylosome protein 50
  • Scientific Description
    The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WDR77 using anti-WDR77 antibody (A04894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR77 antigen affinity purified polyclonal antibody (Catalog # A04894-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR77 at approximately 42 kDa. The expected band size for WDR77 is at 42 kDa.
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