
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody (GTX106197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
WNK1 antibody
GTX106197
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetWNK1
Overview
- SupplierGeneTex
- Product NameWNK1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.18 mg/ml
- ConjugateUnconjugated
- Gene ID65125
- Target nameWNK1
- Target descriptionWNK lysine deficient protein kinase 1
- Target synonymsHSAN2, HSN2, KDP, PPP1R167, PRKWNK1, PSK, p65, serine/threonine-protein kinase WNK1, erythrocyte 65 kDa protein, prostate-derived sterile 20-like kinase, protein kinase with no lysine 1, protein phosphatase 1, regulatory subunit 167, serine/threonine-protein kinase WNK1 1, serine/threonine-protein kinase WNK1 2
- HostRabbit
- IsotypeIgG
- Protein IDQ9H4A3
- Protein NameSerine/threonine-protein kinase WNK1
- Scientific DescriptionThe WNK1 gene encodes a cytoplasmic serine-threonine kinase expressed in distal nephron.[supplied by OMIM]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Ho YJ, Chang J, Yeh KT, et al. Prognostic and Clinical Implications of WNK Lysine Deficient Protein Kinase 1 Expression in Patients With Hepatocellular Carcinoma. In Vivo. 2020,34(5):2631-2640. doi: 10.21873/invivo.12081Read this paper
- Ding L, Zhang L, Biswas S, et al. Akt3 inhibits adipogenesis and protects from diet-induced obesity via WNK1/SGK1 signaling. JCI Insight. 2017,2(22):pii: 95687. doi: 10.1172/jci.insight.95687.Read this paper
- Liu Y, Song X, Shi Y, et al. WNK1 activates large-conductance Ca2+-activated K+ channels through modulation of ERK1/2 signaling. J Am Soc Nephrol. 2015,26(4):844-54. doi: 10.1681/ASN.2014020186Read this paper





![WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. WNK1 stained by WNK1 antibody [HL2184] (GTX638188) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638188/GTX638188_T-44935_20230217_IHC-P_R_23030219_792.webp)





