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WNK1 Antibody (YA2213, Rabbit)

HY-P82468
MedChem Express
TargetWNK1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    WNK1 Antibody (YA2213, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID65125
  • Target name
    WNK1
  • Target description
    WNK lysine deficient protein kinase 1
  • Target synonyms
    erythrocyte 65 kDa protein; HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1; prostate-derived sterile 20-like kinase; protein kinase with no lysine 1; protein phosphatase 1, regulatory subunit 167; PSK; serine/threonine-protein kinase WNK1; serine/threonine-protein kinase WNK1 1; serine/threonine-protein kinase WNK1 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    WNK1 Antibody (YA2213) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2213), targeting WNK1, with a predicted molecular weight of 251 kDa (observed band size: 251 kDa). WNK1 Antibody (YA2213) can be used for WB, IHC-P experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WNK1 using anti-WNK1 antibody (PB9964). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNK1 antigen affinity purified polyclonal antibody (Catalog # PB9964) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNK1 at approximately 250 kDa. The expected band size for WNK1 is at 251 kDa.
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