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Immunofluorescent staining of human cell line A549 shows localization to cytosol.
Immunofluorescent staining of human cell line A549 shows localization to cytosol.
Immunofluorescent staining of human cell line A549 shows localization to cytosol.

Anti-WNK1 Antibody

HPA046541
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetWNK1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WNK1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID65125
  • Target name
    WNK1
  • Target description
    WNK lysine deficient protein kinase 1
  • Target synonyms
    HSAN2, HSN2, KDP, PPP1R167, PRKWNK1, PSK, p65, serine/threonine-protein kinase WNK1, erythrocyte 65 kDa protein, prostate-derived sterile 20-like kinase, protein kinase with no lysine 1, protein phosphatase 1, regulatory subunit 167, serine/threonine-protein kinase WNK1 1, serine/threonine-protein kinase WNK1 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H4A3
  • Protein Name
    Serine/threonine-protein kinase WNK1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of WNK1 using anti-WNK1 antibody (PB9964). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNK1 antigen affinity purified polyclonal antibody (Catalog # PB9964) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNK1 at approximately 250 kDa. The expected band size for WNK1 is at 251 kDa.
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