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IHC-P analysis of human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) using GTX83266 WNT5A antibody [3D10].
IHC-P analysis of human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) using GTX83266 WNT5A antibody [3D10].
IHC-P analysis of human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) using GTX83266 WNT5A antibody [3D10].

Wnt5a antibody [3D10]

GTX83266
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetWNT5A
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Overview

  • Supplier
    GeneTex
  • Product Name
    Wnt5a antibody [3D10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3D10
  • Conjugate
    Unconjugated
  • Gene ID7474
  • Target name
    WNT5A
  • Target description
    Wnt family member 5A
  • Target synonyms
    epididymis secretory sperm binding protein; hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP41221
  • Protein Name
    Protein Wnt-5a
  • Scientific Description
    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WNT5A using anti-WNT5A antibody (A00549-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT5A antigen affinity purified polyclonal antibody (Catalog # A00549-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT5A at approximately 43 kDa. The expected band size for WNT5A is at 42 kDa.
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