Bio-Connect

Wnt5a antibody

GTX111187
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetWNT5A
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    Wnt5a antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.09 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7474
  • Target name
    WNT5A
  • Target description
    Wnt family member 5A
  • Target synonyms
    epididymis secretory sperm binding protein; hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41221
  • Protein Name
    Protein Wnt-5a
  • Scientific Description
    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cardiac Wnt5a and Wnt11 promote fibrosis by the crosstalk of FZD5 and EGFR signaling under pressure overload. Zou Y et al., 2021 Sep 25, Cell Death Dis
    Read more
  • Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice. Guo CH et al., 2021 Mar 29, Mar Drugs
    Read more
  • Raloxifene inhibits adipose tissue inflammation and adipogenesis through Wnt regulation in ovariectomized rats and 3 T3-L1 cells. Shen HH et al., 2019 Aug 31, J Biomed Sci
    Read more
  • Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a. Gong W et al., 2019 Jan, J Cell Biochem
    Read more
  • Caveolin-1 down-regulation is required for Wnt5a-Frizzled 2 signalling in Ha-RasV12 -induced cell transformation. Lin HK et al., 2018 May, J Cell Mol Med
    Read more
  • Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer. Wang LT et al., 2017 Aug, Oncogene
    Read more
  • A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways. Huang T et al., 2016 Oct 4, Nat Commun
    Read more
  • Paracrine WNT5A Signaling Inhibits Expansion of Tumor-Initiating Cells. Borcherding N et al., 2015 May 15, Cancer Res
    Read more

Related products

Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetWNT5A
  • SizePrice
Product group Antibodies
Assay Genie
TargetWNT5A
  • SizePrice
Anti-Wnt5a Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
  • SizePrice
ICC/IF analysis of PC12 cells using GTX83127 WNT5A antibody [6F2]. Green : WNT5A Blue: DRAQ5 fluorescent DNA dye
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetWNT5A
  • SizePrice
IHC-P analysis of human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) using GTX83266 WNT5A antibody [3D10].
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetWNT5A
  • SizePrice
Product group Antibodies
MedChem Express
TargetWNT5A
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetWNT5A
  • SizePrice
Western Blot Positive WB detected in: Mouse heart tissue All lanes: WNT5A antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 41 kDa Observed band size: 43 kDa
Product group Antibodies
Cusabio
WNT5A AntibodyCSB-PA026138LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetWNT5A
  • SizePrice
Figure 1. Western blot analysis of WNT5A using anti-WNT5A antibody (A00549-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT5A antigen affinity purified polyclonal antibody (Catalog # A00549-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT5A at approximately 43 kDa. The expected band size for WNT5A is at 42 kDa.
Product group Antibodies
Boster Bio
Anti-WNT5A Antibody Picoband(r)A00549-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetWNT5A
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice