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ProductsBack/XIAP Antibody (clone 2D2-C3-D12, Preservative Free)

XIAP Antibody (clone 2D2-C3-D12, Preservative Free)

LS-C743654
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetXIAP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    XIAP Antibody (clone 2D2-C3-D12, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:320000), WB (1:1000 - 1:5000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2D2-C3-D12
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID331
  • Target name
    XIAP
  • Target description
    X-linked inhibitor of apoptosis
  • Target synonyms
    API3; baculoviral IAP repeat-containing protein 4; BIRC4; E3 ubiquitin-protein ligase XIAP; hIAP3; hIAP-3; IAP-3; IAP-like protein 1; ILP1; inhibitor of apoptosis protein 3; MIHA; RING-type E3 ubiquitin transferase XIAP; X-linked IAP; X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase; XLP2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of XIAP using anti-XIAP antibody (PB9965). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XIAP antigen affinity purified polyclonal antibody (Catalog # PB9965) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XIAP at approximately 55 kDa. The expected band size for XIAP is at 57 kDa.
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