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Western Blot analysis of 293 cells using XIAP Polyclonal Antibody
Western Blot analysis of 293 cells using XIAP Polyclonal Antibody
Western Blot analysis of 293 cells using XIAP Polyclonal Antibody

XIAP Antibody

CSB-PA004519
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetXIAP
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Overview

  • Supplier
    Cusabio
  • Product Name
    XIAP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID331
  • Target name
    XIAP
  • Target description
    X-linked inhibitor of apoptosis
  • Target synonyms
    API3; baculoviral IAP repeat-containing protein 4; BIRC4; E3 ubiquitin-protein ligase XIAP; hIAP3; hIAP-3; IAP-3; IAP-like protein 1; ILP1; inhibitor of apoptosis protein 3; MIHA; RING-type E3 ubiquitin transferase XIAP; X-linked IAP; X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase; XLP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP98170
  • Protein Name
    E3 ubiquitin-protein ligase XIAP
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with XIAP Polyclonal Antibody, Unconjugated (bs-55223R) at 1:100 overnight at 4°C, DAB staining.
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Figure 1. Western blot analysis of XIAP using anti-XIAP antibody (PB9965). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XIAP antigen affinity purified polyclonal antibody (Catalog # PB9965) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XIAP at approximately 55 kDa. The expected band size for XIAP is at 57 kDa.
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