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XPC Polyclonal Antibody

BS-25269R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetXPC
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Overview

  • Supplier
    Bioss
  • Product Name
    XPC Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7508
  • Target name
    XPC
  • Target description
    XPC complex subunit, DNA damage recognition and repair factor
  • Target synonyms
    RAD4, XP3, XPCC, p125, DNA repair protein complementing XP-C cells, mutant xeroderma pigmentosum group C, xeroderma pigmentosum, complementation group C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01831
  • Protein Name
    DNA repair protein complementing XP-C cells
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of XPC using anti-XPC antibody (A00473-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPC antigen affinity purified polyclonal antibody (Catalog # A00473-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPC at approximately 106KD. The expected band size for XPC is at 106KD.
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