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Immunofluorescence analysis of A549 cells using XRCC2 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using XRCC2 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using XRCC2 Polyclonal Antibody

XRCC2 Polyclonal Antibody

E-AB-64766
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    XRCC2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of XRCC2 using anti-XRCC2 antibody (A02138-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC2 antigen affinity purified polyclonal antibody (Catalog # A02138-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC2 at approximately 32KD. The expected band size for XRCC2 is at 32KD.
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