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IHC-P analysis of human intestinal cancer tissue using GTX01029 XRCC4 antibody. Dilution : 1microg/ml
IHC-P analysis of human intestinal cancer tissue using GTX01029 XRCC4 antibody. Dilution : 1microg/ml
IHC-P analysis of human intestinal cancer tissue using GTX01029 XRCC4 antibody. Dilution : 1microg/ml

XRCC4 antibody

GTX01029
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetXRCC4
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Overview

  • Supplier
    GeneTex
  • Product Name
    XRCC4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. IHC-P: 0.5-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7518
  • Target name
    XRCC4
  • Target description
    X-ray repair cross complementing 4
  • Target synonyms
    DNA repair protein XRCC4; SSMED; X-ray repair complementing defective repair in Chinese hamster cells 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13426
  • Protein Name
    DNA repair protein XRCC4
  • Scientific Description
    The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site. [provided by RefSeq, Oct 2019]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC4 antigen affinity purified polyclonal antibody (Catalog # A00787-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC4 at approximately 55 kDa. The expected band size for XRCC4 is at 38 kDa.
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