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ProductsBack/XRCC4 Mouse Monoclonal Antibody [Clone ID: OTI4H9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY XRCC4 (Cat# RC218029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC4(Cat# TA500650). Positive lysates [LY402930] (100ug) and [LC402930] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY XRCC4 (Cat# RC218029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC4(Cat# TA500650). Positive lysates [LY402930] (100ug) and [LC402930] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY XRCC4 (Cat# RC218029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC4(Cat# TA500650). Positive lysates [LY402930] (100ug) and [LC402930] (20ug) can be purchased separately from OriGene.

XRCC4 Mouse Monoclonal Antibody [Clone ID: OTI4H9]

CF500650
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetXRCC4
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    XRCC4 Mouse Monoclonal Antibody [Clone ID: OTI4H9]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4H9
  • Gene ID7518
  • Target name
    XRCC4
  • Target description
    X-ray repair cross complementing 4
  • Target synonyms
    SSMED, hXRCC4, DNA repair protein XRCC4, X-ray repair complementing defective repair in Chinese hamster cells 4
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13426
  • Protein Name
    DNA repair protein XRCC4
  • Scientific Description
    Carrier-free (BSA/glycerol-free) XRCC4 mouse monoclonal antibody, clone OTI4H9 (formerly 4H9)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC4 antigen affinity purified polyclonal antibody (Catalog # A00787-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC4 at approximately 55 kDa. The expected band size for XRCC4 is at 38 kDa.
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