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Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ZEB2 Polyclonal Antibody at dilution 1:60
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ZEB2 Polyclonal Antibody at dilution 1:60
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ZEB2 Polyclonal Antibody at dilution 1:60

ZEB2 Polyclonal Antibody

E-AB-10589
Elabscience
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetZEB2
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Overview

  • Supplier
    Elabscience
  • Product Name
    ZEB2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9839
  • Target name
    ZEB2
  • Target description
    zinc finger E-box binding homeobox 2
  • Target synonyms
    HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B, zinc finger E-box-binding homeobox 2, SMAD interacting protein 1, Smad-interacting protein 1, zinc finger homeobox 1b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60315
  • Protein Name
    Zinc finger E-box-binding homeobox 2
  • Scientific Description
    The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 3. Flow Cytometry analysis of U937 cells using anti-ZEB2 antibody (PB9236). Overlay histogram showing U937 cells stained with PB9236 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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