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ZIC1/ZIC2/ZIC3 Antibody

PACO05328
Assay Genie
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetZIC1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    ZIC1/ZIC2/ZIC3 Antibody (PACO05328)
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHC, IF, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID7545
  • Target name
    ZIC1
  • Target description
    Zic family member 1
  • Target synonyms
    BAIDCS; CRS6; ZIC; Zic family member 1 (odd-paired homolog, Drosophila); zinc finger protein 201; Zinc finger protein of the cerebellum 1; zinc finger protein ZIC 1; ZNF201
  • Host
    Rabbit
  • Protein IDO60481
  • Protein Name
    Zinc finger protein ZIC 3
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZIC1 using anti-ZIC1 antibody (A05537-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC1 antigen affinity purified polyclonal antibody (Catalog # A05537-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIC1 at approximately 48 kDa. The expected band size for ZIC1 is at 48 kDa.
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