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ProductsBack/ZIC1/ZIC2/ZIC3/ZIC4/ZIC5 Antibody

ZIC1/ZIC2/ZIC3/ZIC4/ZIC5 Antibody

PACO05329
Assay Genie
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetZIC1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    ZIC1/ZIC2/ZIC3/ZIC4/ZIC5 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    IF, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID7545
  • Target name
    ZIC1
  • Target description
    Zic family zinc finger 1
  • Target synonyms
    BAIDCS, CRS6, ZIC, ZNF201, zinc finger protein ZIC 1, Zic family member 1 (odd-paired homolog, Drosophila), Zinc finger protein of the cerebellum 1, zinc finger protein 201
  • Host
    Rabbit
  • Protein IDO60481
  • Protein Name
    Zinc finger protein ZIC 3
  • Scientific Description
    ZIC1/ZIC2/ZIC3/ZIC4/ZIC5 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for IF, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human, Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human, Mouse
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ZIC1 using anti-ZIC1 antibody (A05537-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC1 antigen affinity purified polyclonal antibody (Catalog # A05537-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIC1 at approximately 48 kDa. The expected band size for ZIC1 is at 48 kDa.
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