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ProductsBack/ZNRD2 Mouse Monoclonal Antibody [Clone ID: OTI2F5]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SSSCA1 (Cat# RC201946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1(Cat# TA501915). Positive lysates [LY416665] (100ug) and [LC416665] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SSSCA1 (Cat# RC201946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1(Cat# TA501915). Positive lysates [LY416665] (100ug) and [LC416665] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SSSCA1 (Cat# RC201946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1(Cat# TA501915). Positive lysates [LY416665] (100ug) and [LC416665] (20ug) can be purchased separately from OriGene.

ZNRD2 Mouse Monoclonal Antibody [Clone ID: OTI2F5]

CF501915
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZNRD2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    ZNRD2 Mouse Monoclonal Antibody [Clone ID: OTI2F5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2F5
  • Gene ID10534
  • Target name
    ZNRD2
  • Target description
    zinc ribbon domain containing 2
  • Target synonyms
    SSSCA1, p27, protein ZNRD2, Sjogren syndrome/scleroderma autoantigen 1, Sjogren's syndrome/scleroderma autoantigen 1, autoantigen p27, centromeric autoantigen (27kD), protein zinc ribbon domain type 2, sjoegren syndrome/scleroderma autoantigen 1, zinc ribbon domain-containing protein 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO60232
  • Protein Name
    Protein ZNRD2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SSSCA1 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SSSCA1/ZNRD2 using anti-SSSCA1/ZNRD2 antibody (A14259-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSSCA1/ZNRD2 antigen affinity purified polyclonal antibody (Catalog # A14259-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSSCA1/ZNRD2 at approximately 23 kDa. The expected band size for SSSCA1/ZNRD2 is at 21 kDa.
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