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ZWINT Antibody

CAB6328
Assay Genie
TargetZWINT
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    ZWINT Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID11130
  • Target name
    ZWINT
  • Target description
    ZW10 interacting kinetochore protein
  • Target synonyms
    human ZW10 interacting protein-1; HZwint-1; KNTC2AP; SIP30; SNAP25 interacting protein of 30 kDa; ZW10 interactor; ZW10 interactor, kinetochore protein; ZWINT1; zwint-1
  • Host
    Rabbit
  • Protein IDO95229
  • Protein Name
    ZW10 interactor
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZWINT using anti-ZWINT antibody (PB10018). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZWINT antigen affinity purified polyclonal antibody (Catalog # PB10018) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZWINT at approximately 31 kDa. The expected band size for ZWINT is at 31 kDa.
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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA030465(ZWINT Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)
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