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Chemical Structure
Chemical Structure
Chemical Structure

2,7-Dichlorofluorescin diacetate

CDX-D0064
Chemodex
CAS Number4091-99-0
Product group Chemicals
Estimated Purity>95%
Molecular Weight487.29
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Overview

  • Supplier
    Chemodex
  • Product Name
    2,7-Dichlorofluorescein diacetate [4091-99-0]
  • Delivery Days Customer
    10
  • CAS Number
    4091-99-0
  • Certification
    Research Use Only
  • Estimated Purity
    >95%
  • Molecular Formula
    C24H16Cl2O7
  • Molecular Weight
    487.29
  • Scientific Description
    Chemical. CAS: 4091-99-0. Formula: C24H16Cl2O7. MW: 487.29. Dichlorofluorescin diacetate (H2DCFDA) is a chemically reduced analog of fluorescein, used as an indicator for reactive oxygen species (ROS) in cells. The cell permeable non-fluorescent H2DCFDA is cleaved by cellular esterases and oxidation, and converted to the highly fluorescent 2,7-dichlorofluorescein. H2DCFDA might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. This probe is widely used to monitoring cellular redox processes. Used as a component/substrate of the 2,7-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS). Can detect reactive oxygen species in live cells. Spectral data: lambdaEx=504nm/lambdaEm=529nm. - Dichlorofluorescin diacetate (H2DCFDA) is a chemically reduced analog of fluorescein, used as an indicator for reactive oxygen species (ROS) in cells. The cell permeable non-fluorescent H2DCFDA is cleaved by cellular esterases and oxidation, and converted to the highly fluorescent 2,7-dichlorofluorescein. H2DCFDA might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. This probe is widely used to monitoring cellular redox processes. Used as a component/substrate of the 2,7-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS). Can detect reactive oxygen species in live cells. Spectral data: lambdaEx=504nm/lambdaEm=529nm.
  • SMILES
    ClC1=C(OC(C)=O)C=C2C(C(C3=CC=CC=C3C(O)=O)C(C=C(Cl)C(OC(C)=O)=C4)=C4O2)=C1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116134

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