Detection of 5-Methylcytosine in mouse different tissues genomic DNA. Sandwich ELISA using anti-5-Methylcytosine (clone RM231) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP-conjugated streptavidin.
Detection of 5-Methylcytosine in mouse different tissues genomic DNA. Sandwich ELISA using anti-5-Methylcytosine (clone RM231) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP-conjugated streptavidin.
5-Methylcytosine (5-mC) DNA Matched Antibody Pair
REV-31-1110-MK
Product group Antibodies
Overview
- SupplierRevMAb Biosciences
- Product Name5-Methylcytosine (5-mC) DNA Matched Antibody Pair
- Delivery Days Customer10
- CertificationResearch Use Only
- Scientific Description5-Methylcytosine is a methylated form of the DNA base cytosine that regulates gene transcription and is involved in epigenetics. It is one of the best known epigenetic modifications. This methylation typically occurs at cytosine in CpG dinucleotides in vertebrates. When cytosine is methylated, the DNA maintains the same sequence, but the expression of methylated genes can be altered. 5-Methylcytosine is a very important repressor of transcription in the genome. When present in promoters, 5-Methylcytosine is associated with stable, long-term transcriptional silencing. This may occur by either blocking positive transcription factors, or promoting the binding of negative ones. - Matched Pair Detection Set. This antibody pair detects 5-methylcytosine in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-hmC, 5-caC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
