53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 protein stained by 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
![53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 53BP1 stained by 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102595/GTX102595_39848_20190111_IHC-P_w_23060119_607.webp "53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 53BP1 stained by 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102595/GTX102595_39848_20161103_WB_shRNA_watermark_w_23060119_680.webp "Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.")
![53BP1 antibody [N1], N-term detects TP53BP1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract 5 % SDS-PAGE 53BP1 antibody [N1], N-term (GTX102595) dilution: 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX102595/GTX102595_39848_WB_w_23060119_151.webp "53BP1 antibody [N1], N-term detects TP53BP1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract 5 % SDS-PAGE 53BP1 antibody [N1], N-term (GTX102595) dilution: 1:500")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102595/GTX102595_39848_20160128_WB_M_w_23060119_344.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.")
53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 protein stained by 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
53BP1 antibody [N1], N-term
GTX102595
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTP53BP1
Overview
- SupplierGeneTex
- Product Name53BP1 antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7158
- Target nameTP53BP1
- Target descriptiontumor protein p53 binding protein 1
- Target synonyms53BP1, TDRD30, p202, p53BP1, TP53-binding protein 1, p53-binding protein 1, tumor protein 53-binding protein, 1, tumor suppressor p53-binding protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ12888
- Protein NameTP53-binding protein 1
- Scientific DescriptionMay have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Uyeda A, Onishi K, Hirayama T, et al. Suppression of DNA Double-Strand Break Formation by DNA Polymerase β in Active DNA Demethylation Is Required for Development of Hippocampal Pyramidal Neurons. J Neurosci. 2020,40(47):9012-9027. doi: 10.1523/JNEUROSCI.0319-20.2020Read this paper
- Onishi K, Uyeda A, Shida M, et al. Genome Stability by DNA Polymerase β in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development. J Neurosci. 2017,37(35):8444-8458. doi: 10.1523/JNEUROSCI.0665-17.2017Read this paper
- Chang HY, Shih MH, Huang HC, et al. Middle infrared radiation induces G2/M cell cycle arrest in A549 lung cancer cells. PLoS One. 2013,8(1):e54117. doi: 10.1371/journal.pone.0054117Read this paper
Datasheet
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