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ProductsBack/ABCC3 Antibody - middle region (OAAB00150)

ABCC3 Antibody - middle region (OAAB00150)

OAAB00150
Aviva Systems Biology
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetABCC3
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    ABCC3 Antibody - middle region (OAAB00150)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid; PBS with 0.09% (W/V) sodium azide.
  • Gene ID8714
  • Target name
    ABCC3
  • Target description
    ATP binding cassette subfamily C member 3
  • Target synonyms
    ABC31, EST90757, MLP2, MOAT-D, MRP3, cMOAT2, ATP-binding cassette sub-family C member 3, ATP-binding cassette, sub-family C (CFTR/MRP), member 3, canalicular multispecific organic anion transporter 2, canicular multispecific organic anion transporter, multi-specific organic anion transporter D, multidrug resistance associated protein, multidrug resistance-associated protein 3
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP3/ABCC3 antigen affinity purified polyclonal antibody (Catalog # A02429-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP3/ABCC3 at approximately 200 kDa. The expected band size for MRP3/ABCC3 is at 169 kDa.
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