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ProductsBack/Actin antibody [AC-40]
IHC-Fr analysis of human placenta tissue using GTX10763 Actin antibody [AC-40].
IHC-Fr analysis of human placenta tissue using GTX10763 Actin antibody [AC-40].
IHC-Fr analysis of human placenta tissue using GTX10763 Actin antibody [AC-40].

Actin antibody [AC-40]

GTX10763
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityChicken, Human, Mouse, Rat
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Overview

  • Supplier
    GeneTex
  • Product Name
    Actin antibody [AC-40]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 2microg/ml. IHC-Fr: 4microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AC-40
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Chicken, Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Actin using anti-Actin antibody (PB9751). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: human Hela whole cell lysates, Lane 9: human HepG2 whole cell lysates, Lane 10: human 293T whole cell lysates, Lane 11: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Actin antigen affinity purified polyclonal antibody (Catalog # PB9751) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Actin at approximately 42 kDa. The expected band size for Actin is at 42 kDa.
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