ICC/IF analysis of Rat2 cells using GTX11003 Actin antibody [AC-40] at 1:250 (red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.
![IHC-Fr analysis of human tongue tissue using GTX11003 Actin antibody [AC-40].](https://www.genetex.com/upload/website/prouct_img/normal/GTX11003/GTX11003_20170605_IHC-Fr_w_23060500_814.webp "IHC-Fr analysis of human tongue tissue using GTX11003 Actin antibody [AC-40].")
![WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK, and (9) MDCK lysates using Actin antibody [AC-40] at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX11003/GTX11003_20170605_WB_w_23060500_778.webp "WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK, and (9) MDCK lysates using Actin antibody [AC-40] at 1:500.")
ICC/IF analysis of Rat2 cells using GTX11003 Actin antibody [AC-40] at 1:250 (red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.
Actin antibody [AC-40]
GTX11003
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityBovine, Canine, Chicken, Fish, Guinea Pig, Goat, Hamster, Human, Monkey, Molluscs, Mouse, Porcine, Rabbit, Reptile, Rat, Sheep, Xenopus
TargetACTA1
Overview
- SupplierGeneTex
- Product NameActin antibody [AC-40]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. ICC/IF: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAC-40
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID58
- Target nameACTA1
- Target descriptionactin alpha 1, skeletal muscle
- Target synonymsACTA; actin, alpha skeletal muscle; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; nemaline myopathy type 3; SHPM
- HostMouse
- IsotypeIgG2a
- Protein IDP68133
- Protein NameActin, alpha skeletal muscle
- Scientific DescriptionThe two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
- ReactivityBovine, Canine, Chicken, Fish, Guinea Pig, Goat, Hamster, Human, Monkey, Molluscs, Mouse, Porcine, Rabbit, Reptile, Rat, Sheep, Xenopus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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- Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration. Ma YX et al., 2022 Mar, Bioact MaterRead more
- Activity of Lymphostatin, A Lymphocyte Inhibitory Virulence Factor of Pathogenic Escherichia coli, is Dependent on a Cysteine Protease Motif. Bease AG et al., 2021 Sep 17, J Mol BiolRead more
- MicroRNA-301a-3p promotes triple-negative breast cancer progression through downregulating MEOX2. Liu H et al., 2021 Sep, Exp Ther MedRead more
- Silencing DNA Polymerase beta Induces Aneuploidy as a Biomarker of Poor Prognosis in Oral Squamous Cell Cancer. Wang HC et al., 2021 Feb 27, Int J Mol SciRead more
- Mcl-1-mediated mitochondrial fission protects against stress but impairs cardiac adaptation to exercise. Moyzis AG et al., 2020 Sep, J Mol Cell CardiolRead more
- Arginine methylation of APE1 promotes its mitochondrial translocation to protect cells from oxidative damage. Zhang Y et al., 2020 Oct, Free Radic Biol MedRead more
- Astrocytes influence medulloblastoma phenotypes and CD133 surface expression. Gronseth E et al., 2020, PLoS OneRead more
- Levetiracetam Protects Against Cognitive Impairment of Subthreshold Convulsant Discharge Model Rats by Activating Protein Kinase C (PKC)-Growth-Associated Protein 43 (GAP-43)-Calmodulin-Dependent Protein Kinase (CaMK) Signal Transduction Pathway. Wang MJ et al., 2019 Jun 22, Med Sci MonitRead more
- Metastatic Colorectal Cancer Rewrites Metabolic Program Through a Glut3-YAP-dependent Signaling Circuit. Kuo CC et al., 2019, TheranosticsRead more