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ACVR2B antibody

70R-35147
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    ACVR2B antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Lane 1:K562 cell lysates; Lane 2: 293T cell lysates probed with ACVR2B/ACTR2B Polyclonal Antibody, Unconjugated (bs-12417R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA206692(ACVR2B Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of ACVR2B using anti-ACVR2B antibody (PB9975). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR2B antigen affinity purified polyclonal antibody (Catalog # PB9975) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR2B at approximately 55 kDa. The expected band size for ACVR2B is at 58 kDa.
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