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Acyl Carrier Protein, Mitochondrial (ACP) Polyclonal Antibody

CAU28057
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetNDUFAB1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Acyl Carrier Protein, Mitochondrial (ACP) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4706
  • Target name
    NDUFAB1
  • Target description
    NADH:ubiquinone oxidoreductase subunit AB1
  • Target synonyms
    ACP; ACP1; acyl carrier protein, mitochondrial; CI-SDAP; complex I SDAP subunit; FASN2A; mitochondrial acyl carrier protein; NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa; NADH:ubiquinone oxidoreductase SDAP subunit; NADH-ubiquinone oxidoreductase 9.6 kDa subunit; SDAP
  • Host
    Rabbit
  • Protein IDO14561
  • Protein Name
    Acyl carrier protein, mitochondrial
  • Scientific Description
    The Acyl Carrier Protein, Mitochondrial (ACP) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFAB1 using anti-NDUFAB1 antibody (M09620). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat RH35 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFAB1 antigen affinity purified monoclonal antibody (Catalog # M09620) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFAB1 at approximately 14 kDa. The expected band size for NDUFAB1 is at 17 kDa.
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