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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA446344(NDUFAB1 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA446344(NDUFAB1 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA446344(NDUFAB1 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)

NDUFAB1 Antibody

CSB-PA446344
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNDUFAB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NDUFAB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4706
  • Target name
    NDUFAB1
  • Target description
    NADH:ubiquinone oxidoreductase subunit AB1
  • Target synonyms
    ACP, ACP1, FASN2A, SDAP, acyl carrier protein, mitochondrial, CI-SDAP, NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa, NADH-ubiquinone oxidoreductase 9.6 kDa subunit, NADH:ubiquinone oxidoreductase SDAP subunit, complex I SDAP subunit, mitochondrial acyl carrier protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14561
  • Protein Name
    Acyl carrier protein, mitochondrial
  • Scientific Description
    NDUFAB1 (NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1), also known as SDAP, ACP (acyl carrier protein) or FASN2A, is one of about 45 subunits comprising Complex I of the oxidative phosphorylation electron transport chain. Consisting of 156 amino acids and localizing to mitochondria, NDUFAB1 functions as an accessory subunit of the multi-protein mitochondrial membrane respiratory chain NADH dehydrogenase complex (known as Complex I). Complex I plays an important role in the transfer of electrons from NADH to the respiratory chain, a process that is essential for cellular respiration. NDUFAB1 contains one acyl carrier domain and is encoded by a gene that maps to human chromosome 16p12.2 and mouse chromosome 7 F3.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFAB1 using anti-NDUFAB1 antibody (M09620). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat RH35 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFAB1 antigen affinity purified monoclonal antibody (Catalog # M09620) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFAB1 at approximately 14 kDa. The expected band size for NDUFAB1 is at 17 kDa.
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