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Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.

Anti-NDUFAB1 Antibody

HPA054364
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetNDUFAB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDUFAB1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4706
  • Target name
    NDUFAB1
  • Target description
    NADH:ubiquinone oxidoreductase subunit AB1
  • Target synonyms
    ACP; ACP1; acyl carrier protein, mitochondrial; CI-SDAP; complex I SDAP subunit; FASN2A; mitochondrial acyl carrier protein; NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa; NADH:ubiquinone oxidoreductase SDAP subunit; NADH-ubiquinone oxidoreductase 9.6 kDa subunit; SDAP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14561
  • Protein Name
    Acyl carrier protein, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFAB1 using anti-NDUFAB1 antibody (M09620). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat RH35 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFAB1 antigen affinity purified monoclonal antibody (Catalog # M09620) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFAB1 at approximately 14 kDa. The expected band size for NDUFAB1 is at 17 kDa.
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