More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/phospho-ADAM17 (Thr735) Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded human gastric cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ADAM17 (Thr735) Polyclonal Antibody (bs-12477R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human gastric cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ADAM17 (Thr735) Polyclonal Antibody (bs-12477R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human gastric cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ADAM17 (Thr735) Polyclonal Antibody (bs-12477R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

phospho-ADAM17 (Thr735) Polyclonal Antibody

BS-12447R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rat, Sheep
TargetADAM17
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    phospho-ADAM17 (Thr735) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6868
  • Target name
    ADAM17
  • Target description
    ADAM metallopeptidase domain 17
  • Target synonyms
    ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE, disintegrin and metalloproteinase domain-containing protein 17, ADAM metallopeptidase domain 18, TNF-alpha convertase, TNF-alpha convertase enzyme, TNF-alpha converting enzyme, a disintegrin and metalloproteinase 17, cartilage snake venom-like protease, snake venom-like protease, tumor necrosis factor, alpha, converting enzyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Human, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

D1(A12) IgG or D1(A12) Fab inhibits constitutive shedding of TNF-alpha from IGROV1 (human ovarian cancer cell line) into culture medium. Medium was collected after 48 hours of incubation with or without IgGs at 200nM.
Product group Antibodies
AdipoGen Life Sciences
anti-ADAM17 (human), mAb (rec.) (blocking) (D1(A12)) (Fab Fragment) (His)AG-27B-6003PF
ApplicationsFunctional Assay, Neutralisation/Blocking
ReactivityHuman
TargetADAM17
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-CD156b [hz1022C3], Human IgG1, kappaAB04681-10.0
ApplicationsFlow Cytometry, ELISA, Other Application
ReactivityHuman
TargetADAM17
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ADAM17 (M) (322-340) Antibody130-10579
ApplicationsELISA
ReactivityHuman
TargetADAM17
  • SizePrice
Figure 1. Western blot analysis of ADAM17 using anti-ADAM17 antibody (A00604). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM17 antigen affinity purified polyclonal antibody (Catalog # A00604) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM17 at approximately 78KD. The expected band size for ADAM17 is at 97KD.
Product group Antibodies
Boster Bio
Anti-ADAM17 Antibody Picoband(r)A00604-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetADAM17
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-ADAM17 / TACEEB05382
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetADAM17
  • SizePrice
Product group Antibodies
Biomatik
A Disintegrin And Metalloprotease 17 (ADAM17) Polyclonal AntibodyCAU25725
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetADAM17
  • SizePrice
Product group Antibodies
Cusabio
Cleaved-ADAM17 (R215) AntibodyCSB-PA050198
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetADAM17
  • SizePrice
Wild-type (WT) and ADAM17 knockout (KO) HeLa (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
ADAM17 antibody [C2C3], C-termGTX101358
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetADAM17
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ADAM17 / TACE Antibody (clone 4B12-F8-E11, Preservative Free)LS-C343161
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetADAM17
  • SizePrice
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.
Product group Antibodies
Atlas Antibodies
Anti-ADAM17 AntibodyHPA010738
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetADAM17
  • SizePrice