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ProductsBack/Cleaved-ADAM17 (R215) Antibody

Cleaved-ADAM17 (R215) Antibody

CSB-PA050198
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetADAM17
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Overview

  • Supplier
    Cusabio
  • Product Name
    Cleaved-ADAM17 (R215) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6868
  • Target name
    ADAM17
  • Target description
    ADAM metallopeptidase domain 17
  • Target synonyms
    ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE, disintegrin and metalloproteinase domain-containing protein 17, ADAM metallopeptidase domain 18, TNF-alpha convertase, TNF-alpha convertase enzyme, TNF-alpha converting enzyme, a disintegrin and metalloproteinase 17, cartilage snake venom-like protease, snake venom-like protease, tumor necrosis factor, alpha, converting enzyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78536
  • Protein Name
    Disintegrin and metalloproteinase domain-containing protein 17
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ADAM17 using anti-ADAM17 antibody (A00604). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM17 antigen affinity purified polyclonal antibody (Catalog # A00604) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM17 at approximately 78KD. The expected band size for ADAM17 is at 97KD.
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