Wild-type (WT) and ADAM17 knockout (KO) HeLa (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![ADAM17 antibody [C2C3], C-term detects ADAM17 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract | 7.5% SDS-PAGE ADAM17 antibody [C2C3], C-term (GTX101358) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101358/GTX101358_39680_WB_w_23060100_915.webp "ADAM17 antibody [C2C3], C-term detects ADAM17 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract | 7.5% SDS-PAGE ADAM17 antibody [C2C3], C-term (GTX101358) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101358/GTX101358_39680_20151203_WB_R_w_23060100_463.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![ADAM17 antibody [C2C3], C-term detects ADAM17 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: ADAM17 antibody [C2C3], C-term (GTX101358) dilution: 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101358/GTX101358_39680_IHC_w_23060100_928.webp "ADAM17 antibody [C2C3], C-term detects ADAM17 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: ADAM17 antibody [C2C3], C-term (GTX101358) dilution: 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101358/GTX101358_39680_20151203_WB_M_w_23060100_417.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101358/GTX101358_44531_20220422_WB_w_23060100_424.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Wild-type (WT) and ADAM17 knockout (KO) HeLa (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ADAM17 antibody [C2C3], C-term
GTX101358
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetADAM17
Overview
- SupplierGeneTex
- Product NameADAM17 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.21 mg/ml
- ConjugateUnconjugated
- Gene ID6868
- Target nameADAM17
- Target descriptionADAM metallopeptidase domain 17
- Target synonymsADAM18, CD156B, CSVP, NISBD, NISBD1, TACE, disintegrin and metalloproteinase domain-containing protein 17, ADAM metallopeptidase domain 18, TNF-alpha convertase, TNF-alpha convertase enzyme, TNF-alpha converting enzyme, a disintegrin and metalloproteinase 17, cartilage snake venom-like protease, snake venom-like protease, tumor necrosis factor, alpha, converting enzyme
- HostRabbit
- IsotypeIgG
- Protein IDP78536
- Protein NameDisintegrin and metalloproteinase domain-containing protein 17
- Scientific DescriptionThis gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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