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ProductsBack/ADAR2 / ADARB1 Antibody (450-530 aa, Internal)

ADAR2 / ADARB1 Antibody (450-530 aa, Internal)

LS-C381909
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetADARB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ADAR2 / ADARB1 Antibody (450-530 aa, Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:20000), IHC (1:100 - 1:300), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID104
  • Target name
    ADARB1
  • Target description
    adenosine deaminase RNA specific B1
  • Target synonyms
    ADAR2, DRABA2, DRADA2, NEDHYMS, RED1, double-stranded RNA-specific editase 1, RNA editing deaminase 1, RNA-editing enzyme 1, adenosine deaminase, RNA-specific, B1 (homolog of rat RED1), dsRNA adenosine deaminase DRADA2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RED1/ADARB1 using anti-RED1/ADARB1 antibody (A01810-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RED1/ADARB1 antigen affinity purified polyclonal antibody (Catalog # A01810-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RED1/ADARB1 at approximately 90 kDa. The expected band size for RED1/ADARB1 is at 81 kDa.
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