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Western Blot analysis of HepG2 cells using ADAR2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using ADAR2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using ADAR2 Polyclonal Antibody

ADARB1 Antibody

CSB-PA000821
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetADARB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ADARB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID104
  • Target name
    ADARB1
  • Target description
    adenosine deaminase RNA specific B1
  • Target synonyms
    ADAR2; adenosine deaminase, RNA-specific, B1 (homolog of rat RED1); double-stranded RNA-specific editase 1; DRABA2; DRADA2; dsRNA adenosine deaminase DRADA2; NEDHYMS; RED1; RNA editing deaminase 1; RNA-editing enzyme 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78563
  • Protein Name
    Double-stranded RNA-specific editase 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RED1/ADARB1 using anti-RED1/ADARB1 antibody (A01810-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RED1/ADARB1 antigen affinity purified polyclonal antibody (Catalog # A01810-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RED1/ADARB1 at approximately 90 kDa. The expected band size for RED1/ADARB1 is at 81 kDa.
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