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Paraformaldehyde-fixed, paraffin embedded Mouse Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADH5 Polyclonal Antibody, Unconjugated (bs-12448R) at 1:200 overnight at 4°C, followed by secondary antibody DAB and counterstaining.
Paraformaldehyde-fixed, paraffin embedded Mouse Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADH5 Polyclonal Antibody, Unconjugated (bs-12448R) at 1:200 overnight at 4°C, followed by secondary antibody DAB and counterstaining.
Paraformaldehyde-fixed, paraffin embedded Mouse Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADH5 Polyclonal Antibody, Unconjugated (bs-12448R) at 1:200 overnight at 4°C, followed by secondary antibody DAB and counterstaining.

ADH5 Polyclonal Antibody

BS-12448R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetADH5
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Overview

  • Supplier
    Bioss
  • Product Name
    ADH5 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID128
  • Target name
    ADH5
  • Target description
    alcohol dehydrogenase 5 (class III), chi polypeptide
  • Target synonyms
    ADH-3, ADHX, AMEDS, BMFS7, FALDH, FDH, GSH-FDH, GSNOR, HEL-S-60p, alcohol dehydrogenase class-3, S-(hydroxymethyl)glutathione dehydrogenase, S-nitrosoglutathione reductase, alcohol dehydrogenase (class III), chi polypeptide, alcohol dehydrogenase class chi chain, alcohol dehydrogenase class-III, epididymis secretory sperm binding protein Li 60p, formaldehyde dehydrogenase, glutathione-dependent formaldehyde dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11766
  • Protein Name
    Alcohol dehydrogenase class-3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of ADH5 using anti-ADH5 antibody (RP1111). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MOLT4 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH5 antigen affinity purified polyclonal antibody (Catalog # RP1111) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH5 at approximately 39 kDa. The expected band size for ADH5 is at 40 kDa.
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