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ProductsBack/AFAP (AFAP1) Mouse Monoclonal Antibody [Clone ID: OTI4F4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Positive lysates LY404799 (100ug) and LC404799 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Positive lysates LY404799 (100ug) and LC404799 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Positive lysates LY404799 (100ug) and LC404799 (20ug) can be purchased separately from OriGene.

AFAP (AFAP1) Mouse Monoclonal Antibody [Clone ID: OTI4F4]

TA802333
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetAFAP1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    AFAP (AFAP1) Mouse Monoclonal Antibody [Clone ID: OTI4F4]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4F4
  • Gene ID60312
  • Target name
    AFAP1
  • Target description
    actin filament associated protein 1
  • Target synonyms
    AFAP, AFAP-110, AFAP110, actin filament-associated protein 1, 110 kDa actin filament-associated protein, actin filament-associated protein, 110 kDa
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ8N556
  • Protein Name
    Actin filament-associated protein 1
  • Scientific Description
    AFAP1 mouse monoclonal antibody, clone OTI4F4 (formerly 4F4)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of AFAP/AFAP1 using anti-AFAP/AFAP1 antibody (A05258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: rat stomach tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse lung tissue lysates, Lane 10: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFAP/AFAP1 antigen affinity purified polyclonal antibody (Catalog # A05258-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AFAP/AFAP1 at approximately 120KD. The expected band size for AFAP/AFAP1 is at 120KD.
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