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ProductsBack/AGPAT2 antibody, C-term
WB analysis of Jurkat cells using GTX46672 AGPAT2 antibody at 5.0microg/ml. Lane A : marker Lane B : Jurkat cells
WB analysis of Jurkat cells using GTX46672 AGPAT2 antibody at 5.0microg/ml. Lane A : marker Lane B : Jurkat cells
WB analysis of Jurkat cells using GTX46672 AGPAT2 antibody at 5.0microg/ml. Lane A : marker Lane B : Jurkat cells

AGPAT2 antibody, C-term

GTX46672
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetAGPAT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    AGPAT2 antibody, C-term
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10555
  • Target name
    AGPAT2
  • Target description
    1-acylglycerol-3-phosphate O-acyltransferase 2
  • Target synonyms
    1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta); 1-acyl-sn-glycerol-3-phosphate acyltransferase beta; 1-AGP acyltransferase 2; 1-AGPAT 2; 1-AGPAT2; BSCL; BSCL1; LPAAB; LPAAT-beta; lysophosphatidic acid acyltransferase-beta; testicular tissue protein Li 143
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15120
  • Protein Name
    1-acyl-sn-glycerol-3-phosphate acyltransferase beta
  • Scientific Description
    This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AGPAT2 using anti-AGPAT2 antibody (A04744-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGPAT2 antigen affinity purified polyclonal antibody (Catalog # A04744-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGPAT2 at approximately 33 kDa. The expected band size for AGPAT2 is at 33 kDa.
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