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ProductsBack/Rabbit anti Human Lysophosphatidic Acid Acetyltransferse beta

Rabbit anti Human Lysophosphatidic Acid Acetyltransferse beta

X1652P
Nordic-MUbio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetAGPAT2
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human Lysophosphatidic Acid Acetyltransferse beta
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for Western blotting (5-10 microg/ml) and EIA. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Western Blotting;Enzyme Immunoassay;Western Blotting
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10555
  • Target name
    AGPAT2
  • Target description
    1-acylglycerol-3-phosphate O-acyltransferase 2
  • Target synonyms
    1-AGPAT2, BSCL, BSCL1, LPAAB, LPAAT-beta, LPLAT2, 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, 1-AGP acyltransferase 2, 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta), lysophosphatidic acid acyltransferase-beta, lysophospholipid acyltransferase 2, testicular tissue protein Li 143
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15120
  • Protein Name
    1-acyl-sn-glycerol-3-phosphate acyltransferase beta
  • Scientific Description
    1-AGP acyltransferase 2, 1-AGPAT 2,1-acylglycerol-3-phosphate O-acyltransferase 2, LPAAT-bèta, 2
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Synthetic peptide derived from the LPAAT- protein
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AGPAT2 using anti-AGPAT2 antibody (A04744-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGPAT2 antigen affinity purified polyclonal antibody (Catalog # A04744-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGPAT2 at approximately 33 kDa. The expected band size for AGPAT2 is at 33 kDa.
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